We current the event of a health-monitoring nanofluidic membrane using biocompatible and biodegradable graphene oxide, chitosan, and graphene quantum dots. The nanoconfinement offered by graphene oxide nanolayers encapsulates chitosan molecules, permitting for his or her conformational modifications and switchable hydrophobic-hydrophilic habits in response to pH variations. This low-dimensional membrane operates as an array of nanofluidic channels that may launch quantum dots upon pH modifications. The photoluminescence emission from quantum dots allows fast and dependable optical visualization of pH modifications, facilitating environment friendly human well being monitoring. To make sure fouling prevention and allow a number of usages, we undertake a design method that avoids direct contact between biomarkers and the nanochannels. This design technique, coupled with good mechanical properties (Younger’s modulus of 4.5 GPa), preserves the integrity and performance of the sensors for repeated sensing cycles. Moreover, leveraging the reminiscence impact, our sensors could be reloaded with graphene quantum dots a number of occasions with out vital lack of selectivity, reaching reusability. The wide-ranging capabilities of 2D supplies and stimuli-responsive polymers empower our sustainable method to designing low-dimensional, strong, and versatile sensing supplies. This method permits for the mixing of varied biorecognition components and sign transduction modes, increasing the flexibility and functions of the designed supplies.